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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which ends up in chromosome instability, and thus could contribute to tumorigenesis. This protein was discovered to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in advertising and marketing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up reported but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind particularly to the focus on antibody and block antibody binding. These peptide typically has the epitope recognized because of the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
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This tutorial outlines further more doable optimization of cellular and immunoassay parameters to guarantee the best possible final results are...
Antibodies certain to the blocking peptide no longer bind to the epitope about the goal protein. By comparing the staining from your blocked antibody as opposed to the antibody by itself, you can see which staining is precise.
Focus on Facts Cyclin E belongs on the hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which contribute into the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as here cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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